Maus anti-Human IgD Antikörper

Produktnummer ABIN457925

Kurzübersicht für Maus anti-Human IgD Antikörper (ABIN457925)

Target: IgD

Klonalität: Monoklonal

Applikation: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Klon: NI 158

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. Monoclonal mouse antibody to human IgD, isotype specific

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified monoclonal IgD isolated from human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgD and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Konzentration: 1 mg/mL

Buffer: No foreign proteins added.

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgD

Substanzklasse: Antibody