Maus anti-Human IgA1 Antikörper

Produktnummer ABIN457922

Kurzübersicht für Maus anti-Human IgA1 Antikörper (ABIN457922)

Target: IgA1

Klonalität: Monoklonal

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Haemagglutination (H)

Klon: NI 69-11 (A89-036)

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to a subclass specific determinant on the C H 2 domain of IgA1 as tested in haem- agglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA1, subclass specific

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified monoclonal IgA1 isolated from pooled human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA1, haemagglutination and immunoblotting.
The optimum working dilu-tion is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:3000 to 1:50000. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Konzentration: 1 mg/mL

Buffer: No foreign proteins added.

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgA1