Maus anti-Human IgM Antikörper (FITC)

Produktnummer ABIN457906

Kurzübersicht für Maus anti-Human IgM Antikörper (FITC) (ABIN457906)

Target: IgM

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF)

Klon: NI 179

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific

Produktmerkmale: Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.4.

Aufreinigung: Purified

Immunogen: Highly purified IgM isolated from pooled human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immuno-fluorescence staining of cytoplasmic IgM and cell membrane IgM.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. These data should be interpreted as general recommendations only.

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilize d product by adding 0. 5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgM

Substanzklasse: Antibody