Maus anti-Human IgM Antikörper (Biotin)

Produktnummer ABIN457905

Kurzübersicht für Maus anti-Human IgM Antikörper (Biotin) (ABIN457905)

Target: IgM

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB)

Klon: NI 179

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Biotin-conjugated purified monoclonal mouse antibody to human IgM, isotype specific

Produktmerkmale: Biotin/IgG protein molar ratio (B/P) approximately 7.1.

Aufreinigung: Purified

Immunogen: Highly purified polyclonal IgM isolated from human serum.

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgM

Substanzklasse: Antibody