Maus anti-Human IgD Antikörper (HRP)

Produktnummer ABIN457875

Produktdetails

Target: IgD

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgD, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgD, isotype specific
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Aufreinigung: Purified

Immunogen: Highly purified polyclonal IgD isolated from human serum.

Klon: NI 158

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500, in ELISA from 1:1000 upwards, in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Antigendetails

Target: IgD

Abstract: IgD Produkte

Substanzklasse: Antibody