Maus anti-Human IgG4 (Fc Region) Antikörper (HRP)

Produktnummer ABIN457839

Produktdetails

Target: IgG4

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA

Spezifität: The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG4, subclass specific

Produktmerkmale: Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Aufreinigung: Purified

Immunogen: Highly purified monoclonal IgG4 isolated from pooled human serum

Klon: NI 315 (HP 6206)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Antigendetails

Target: IgG4

Andere Bezeichnung: Immunoglobulin G4 (IgG4) (IgG4 Produkte)

Substanzklasse: Antibody