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Maus anti-Human IgG4 (Fc Region) Antikörper (HRP)

WB, ELISA Monoclonal NI 315 (HP 6206) IgG1 kappa HRP
Produktnummer ABIN457839
  • Target Alle IgG4 Produkte
    IgG4
    Bindungsspezifität
    • 8
    • 7
    • 1
    • 1
    Fc Region
    Reaktivität
    • 34
    • 2
    • 1
    • 1
    Human
    Wirt
    • 26
    • 7
    • 1
    Maus
    Klonalität
    • 28
    • 6
    Monoklonal
    Konjugat
    • 11
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Applikation
    • 22
    • 19
    • 14
    • 10
    • 9
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Spezifität
    The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG4, subclass specific
    Produktmerkmale
    Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
    Aufreinigung
    Purified
    Immunogen
    Highly purified monoclonal IgG4 isolated from pooled human serum
    Klon
    NI 315 (HP 6206)
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting.
    The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    Konzentration
    0.4 mg/mL
    Buffer
    Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
  • Target
    IgG4
    Andere Bezeichnung
    Immunoglobulin G4 (IgG4) (IgG4 Produkte)
    Substanzklasse
    Antibody
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