Kurzübersicht für Maus anti-Human IgM Antikörper (ABIN457833)
Target
IgM
Klonalität
Monoklonal
Applikation
ELISA, Immunofluorescence (IF)
Klon
NI 179
Isotyp
IgG1 kappa
Reaktivität
Human
Wirt
Maus
Spezifität
The reactivity of the antiserum is restricted to an isotype specific determinant on the IgM molecule as tested in ELISA, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cyto-plasmic Ig. Purified monoclonal mouse antibody to human IgM, isotype specific
Produktmerkmale
Purified monoclonal mouse antibody to human IgM, isotype specific
Aufreinigung
Purified
Immunogen
Highly purified monoclonal IgM isolated from pooled human serum
To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgM immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. These data should be interpreted as general recommendations only.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Konzentration
0.8 mg/mL
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Handhabung
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.