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Maus anti-Human IgG4 (Fc Region) Antikörper

WB, ELISA, IF Monoclonal NI 315 (HP 6206) IgG1 kappa unconjugated
Produktnummer ABIN457832
  • Target Alle IgG4 Produkte
    IgG4
    Bindungsspezifität
    • 8
    • 7
    • 1
    • 1
    Fc Region
    Reaktivität
    • 34
    • 2
    • 1
    • 1
    Human
    Wirt
    • 26
    • 7
    • 1
    Maus
    Klonalität
    • 28
    • 6
    Monoklonal
    Applikation
    • 22
    • 19
    • 14
    • 10
    • 9
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Spezifität
    The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG4, subclass specific
    Aufreinigung
    Purified
    Immunogen
    Highly purified monoclonal IgG4 isolated from human serum
    Klon
    NI 315 (HP 6206)
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting.
    The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
    Konzentration
    0.4 mg/mL
    Buffer
    Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
    Konservierungsmittel
    Without preservative
    Handhabung
    Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgG4
    Andere Bezeichnung
    Immunoglobulin G4 (IgG4) (IgG4 Produkte)
    Substanzklasse
    Antibody
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