Maus anti-Human IgG Antikörper

Produktnummer ABIN457826

Kurzübersicht für Maus anti-Human IgG Antikörper (ABIN457826)

Target: IgG

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Klon: NI 335 and NI 343

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. It reacts with the 4 subclasses of human IgG, as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG, isotype specific

Aufreinigung: Purified

Immunogen: Highly purified monoclonal IgG isolated from human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG

Substanzklasse: Antibody