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Maus anti-Human IgG (Fc Region) Antikörper

ELISA, WB, IF Monoclonal NI 335 and NI 343 IgG1 kappa unconjugated

Produktnummer ABIN457826

Datenblatt (PDF)

Target Alle IgG Produkte

IgG
Bindungsspezifität
2356

502

411

188

130

124

94

29

28

28

17

9

6

6

6

6

5

2

2

1

1

1

1
Fc Region
Reaktivität
700

680

570

458

368

239

200

148

140

132

122

98

97

96

94

63

47

38

27

17

12

10

8

5

5

4

3

3

3

3

3

3

3

3

2

2

2

1

1

1

1

1
Human
Wirt
1740

1486

539

219

118

103

33

31

21

17

15

11

11

1
Maus
Klonalität
3369

196

1
Monoklonal
Applikation
1871

1798

1792

1538

988

934

913

515

234

115

115

111

100

87

77

52

50

49

32

27

25

21

17

16

11

8

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1
ELISA, Western Blotting (WB), Immunofluorescence (IF)

Spezifität

The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. It reacts with the 4 subclasses of human IgG, as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG, isotype specific

Aufreinigung

Purified

Immunogen

Highly purified monoclonal IgG isolated from human serum

Klon

NI 335 and NI 343

Isotyp

IgG1 kappa
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (F(ab')2 Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Rabbit anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Goat anti-Human IgG (Fc Region) antibody (HRP)
ELISA, WB Polyclonal HRP F(ab')2 fragment

Mouse anti-Human IgG (Heavy & Light Chain) antibody
ELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated

Applikationshinweise

To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration

0.4 mg/mL

Buffer

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel

Without preservative

Handhabung

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
Target

IgG

Abstract

IgG Produkte

Substanzklasse

Antibody