Kurzübersicht für Maus anti-Human Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antikörper (ABIN457819)
Target
Bence-Jones Protein lambda
Klonalität
Monoklonal
Applikation
ELISA, Western Blotting (WB)
Klon
NI 412 and NI 268
Isotyp
IgG1 kappa
Bindungsspezifität
Chain lambda, Free & Bound
Reaktivität
Human
Wirt
Maus
Produktmerkmale
The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig lambda chain, free and bound
Aufreinigung
Purified
Immunogen
Highly purified Bence Jones lambda proteins isolated from pooled human urine.
Applikationshinweise
To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidasestaining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Konzentration
0.4 mg/mL
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Konservierungsmittel
Without preservative
Handhabung
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.