Maus anti-Human Bence-Jones Protein kappa (Chain kappa), (Free & Bound) Antikörper

Produktnummer ABIN457818

Produktdetails

Target: Bence-Jones Protein kappa

Bindungsspezifität: Chain kappa, Free & Bound

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig kappa chain, free and bound

Aufreinigung: Purified

Immunogen: Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Klon: NI 285 and NI 250

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: Bence-Jones Protein kappa

Andere Bezeichnung: Bence Jones kappa