Schaf anti-Meerschweinchen IgA, IgG, IgM (Heavy & Light Chain) Antikörper

Produktnummer ABIN457776

Kurzübersicht für Schaf anti-Meerschweinchen IgA, IgG, IgM (Heavy & Light Chain) Antikörper (ABIN457776)

Target: IgA, IgG, IgM

Klonalität: Polyklonal

Applikation: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Meerschweinchen

Wirt: Schaf

Spezifität: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins.

Produktmerkmale: Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal guinea pig serum in agar block titration.

Aufreinigung: Delipidated, heat inactivated, lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Purified normal Ig fractions isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal guinea pig serum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Konservierungsmittel: Without preservative

Handhabung: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG + IgA + IgM

Substanzklasse: Antibody