Kaninchen anti-Meerschweinchen IgG2 (Subclass specific) Antikörper

Produktnummer ABIN457771

Kurzübersicht für Kaninchen anti-Meerschweinchen IgG2 (Subclass specific) Antikörper (ABIN457771)

Target: IgG2

Klonalität: Polyklonal

Applikation: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Subclass specific

Reaktivität: Meerschweinchen

Wirt: Kaninchen

Verwendungszweck: Rabbit anti Guinea Pig IgG2 (subclass specific)

Spezifität: Precipitating polyclonal rabbit antiserum to guinea Pig IgG2, subclass specific.

Kreuzreaktivität (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is directed to the Fc subunit of subclass IgG2. It does not react with other IgG1, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgG2 from serum or plasma.

Aufreinigung: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Pools of purified normal IgG2 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added as it may interfere with he antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day

Antigendetails

Target: IgG2

Substanzklasse: Antibody