Maus anti-Human IgE Antikörper (Biotin)

Produktnummer ABIN457502

Kurzübersicht für Maus anti-Human IgE Antikörper (Biotin) (ABIN457502)

Target: IgE

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Klon: BSW17-NBS01

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensitivität: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)

Produktmerkmale: IGHE, chromosome 14q32.33

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Reinheit: > 85 %, tested by analytical Gel Permeation Chromatography

Immunogen: purified human myeloma IgE

Anwendungsinformationen

Applikationshinweise: When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: 4 °C

Antigendetails

Target: IgE

Hintergrund: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.