Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , Sep , Depletion
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C
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Li, Irrazabal, So, Berru, Du, Lam, Ling, Gommerman, Pan-Hammarström, Martin: "The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining." in: Nature communications, Vol. 9, Issue 1, pp. 1006, (2018) (PubMed).