Ziege anti-Human IgA Antikörper (PE)

Produktnummer ABIN376514

Kurzübersicht für Ziege anti-Human IgA Antikörper (PE) (ABIN376514)

Target: IgA

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: PE

Applikation: ELISA, Flow Cytometry (FACS)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Spezifität: Reacts with the heavy chain of human IgA

Produktmerkmale: Goat F(ab')2 Anti-Human IgA-PE

Aufreinigung: Affinity chromatography on sheep IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody.

Anwendungsinformationen

Applikationshinweise:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , Stim
• Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:100 - 1:400 AF555 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen

Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." in: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).

Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." in: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).

Antigendetails

Target: IgA

Substanzklasse: Antibody