Ziege anti-Human IgD Antikörper (PE)

Produktnummer ABIN376508

Kurzübersicht für Ziege anti-Human IgD Antikörper (PE) (ABIN376508)

Target: IgD

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), ELISA

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Spezifität: Reacts with the heavy chain of human IgD

Produktmerkmale: Goat Anti-Human IgD-PE

Aufreinigung: Purification Method: Affinity chromatography on human IgD covalently linked to agarose.

Anwendungsinformationen

Applikationshinweise:

• Applications: Quality tested applications include - ELISA FLISA FC ,
• Other referenced applications include - IHC-FS , IHC-PS , Sep
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen

Han, Jones, Nicely, Reed, Shen, Mansouri, Louder, Trama, Alam, Edwards, Bonsignori, Tomaras, Korber, Montefiori, Mascola, Seaman, Haynes, Saunders: "Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations." in: Nature communications, Vol. 10, Issue 1, pp. 2898, (2019) (PubMed).

Blasi, Negri, LaBranche, Alam, Baker, Brunner, Gladden, Michelini, Vandergrift, Wiehe, Parks, Shen, Bonsignori, Tomaras, Ferrari, Montefiori, Santra, Haynes, Moody, Cara, Klotman: "IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells." in: Communications biology, Vol. 1, pp. 134, (2018) (PubMed).

Williams, Zhang, Jiang, Nicely, Fera, Luo, Moody, Liao, Alam, Kepler, Ramesh, Wiehe, Holland, Bradley, Vandergrift, Saunders, Parks, Foulger, Xia, Bonsignori, Montefiori, Louder, Eaton, Santra et al.: "Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations. ..." in: Nature communications, Vol. 8, Issue 1, pp. 1732, (2018) (PubMed).

Bradley, Pollara, Santra, Vandergrift, Pittala, Bailey-Kellogg, Shen, Parks, Goodman, Eaton, Balachandran, Mach, Saunders, Weiner, Scearce, Sutherland, Phogat, Tartaglia, Reed, Hu, Theis, Pinter et al.: "Pentavalent HIV-1 vaccine protects against simian-human immunodeficiency virus challenge. ..." in: Nature communications, Vol. 8, pp. 15711, (2018) (PubMed).

Hammarlund, Thomas, Amanna, Holden, Slayden, Park, Gao, Slifka: "Plasma cell survival in the absence of B cell memory." in: Nature communications, Vol. 8, Issue 1, pp. 1781, (2018) (PubMed).

Antigendetails

Target: IgD

Substanzklasse: Antibody