Kurzübersicht für Ziege anti-Maus IgG2b (Heavy Chain) Antikörper (HRP) (ABIN376251)
Target
IgG2b
Klonalität
Polyklonal
Konjugat
HRP
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotyp
IgG
Bindungsspezifität
Heavy Chain
Reaktivität
Maus
Wirt
Ziege
Spezifität
Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.
Produktmerkmale
Source: Pooled antisera from goats hyperimmunized with mouse IgG 2b paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Aufreinigung
Affinity chromatography on mouse IgG 3 covalently linked to agarose.
ELISA, FACS
Polyclonal
IgG
Biotin
F(ab')2 fragment
Applikationshinweise
Working Dilution: 1:4,000-1:8,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C
Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).
Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).