Maus anti-Human IgE (Fc Region) Antikörper

Produktnummer ABIN363694

Kurzübersicht für Maus anti-Human IgE (Fc Region) Antikörper (ABIN363694)

Target: IgE

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Klon: Le27-NBS01

Isotyp: IgG1 kappa

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Spezifität: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Produktmerkmale: IGHE, chromosome 14q32.33

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Reinheit: > 95 %, tested by analytical Gel Permeation Chromatography

Sterilität: Sterile filtered

Immunogen: purified human myeloma IgE

Anwendungsinformationen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Konservierungsmittel: Azide free

Handhabung: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Lagerung: 4 °C

Referenzen

Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

Antigendetails

Target: IgE

Hintergrund: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.