Maus anti-Human IgE (Fc Region) Antikörper

Produktnummer ABIN363691

Kurzübersicht für Maus anti-Human IgE (Fc Region) Antikörper (ABIN363691)

Target: IgE

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Klon: Le27-NBS01

Isotyp: IgG1 kappa

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Spezifität: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Produktmerkmale: IGHE, chromosome 14q32.33

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Reinheit: > 95 %, tested by analytical Gel Permeation Chromatography

Immunogen: purified human myeloma IgE

Anwendungsinformationen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Lagerung: 4 °C

Referenzen

Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

Antigendetails

Target: IgE

Hintergrund: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.