Ziege anti-Ratte IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP))
Kurzübersicht für Ziege anti-Ratte IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) (ABIN339133)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- Fc Region
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Reaktivität
- Ratte
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Wirt
- Ziege
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Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F (ab')2 or Bovine, Horse, and Human Serum Proteins.
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Rat IgG F(c) fragment. Label: Alkaline Phosphatase (Calf Intestine) (MW: 140000 daltons).
Type of Immunogen: Purified protein
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Applikationshinweise
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Approved: ELISA (1:1000), IHC, WB
Usage: Suitable for Western Blot, ELISA and Immunohistochemistry. ELISA: 1:1000 to 1:5000 using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Kommentare
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Target Species of Antibody: Rat
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 0.05 M Tris -HCl, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4°C. Do not freeze.
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- IgG
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Substanzklasse
- Antibody
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Molekulargewicht
- 140 kDa
Target
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