Ziege anti-Ratte IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP))
Kurzübersicht für Ziege anti-Ratte IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) (ABIN339103)
Target
Klonalität
Konjugat
Applikation
Isotyp
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Bindungsspezifität
- Fc Region
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Reaktivität
- Ratte
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Wirt
- Ziege
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Spezifität
- Recognizes rat IgG F(c) fragment. Does not cross-react with rat IgG F (ab')2. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rat IgG, rat IgG F(c) and rat serum.
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Rat IgG F(c) fragment.
Type of Immunogen: Purified protein
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Applikationshinweise
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Approved: ELISA (1:1000 - 1:5000), IHC, WB
Usage: Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:1000-1:5000. assayed against 1 μg of rat IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Kommentare
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Target Species of Antibody: Rat
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 4°C. Do not freeze.
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- IgG
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Substanzklasse
- Antibody
Target
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