Ziege anti-Kaninchen IgG (F(ab')2 Region) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN338973

Kurzübersicht für Ziege anti-Kaninchen IgG (F(ab')2 Region) Antikörper (Alkaline Phosphatase (AP)) (ABIN338973)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Kaninchen

Wirt: Ziege

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F (ab')2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.

Aufreinigung: Immunoaffinity purified

Immunogen: Rabbit IgG F (ab')2 fragment.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution (s): This product has been assayed against 1 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:600 to 1:2900 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Rabbit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Antigendetails

Target: IgG

Substanzklasse: Antibody