Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN338473

Kurzübersicht für Ziege anti-Pferd IgG (F(ab')2 Region) Antikörper (Alkaline Phosphatase (AP)) (ABIN338473)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Pferd

Wirt: Ziege

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG, Horse IgG F (ab')2 and Horse Serum. No reaction was observed against Horse IgG F(c).

Aufreinigung: Immunoaffinity purified

Immunogen: Horse IgG F (ab')2 fragment Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140000 daltons).

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1000 - 1:4000), IHC, WB

Usage: Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. ELISA (standard capture): 1:1000-1:4000, 1.0 μg of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Horse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: TBS, 0.001 M Magnesium chloride, 0.0001 M Zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Antigendetails

Target: IgG

Substanzklasse: Antibody

Molekulargewicht: 140 kDa