Kaninchen anti-Human Ig (Chain kappa) Antikörper

Produktnummer ABIN336978

Produktdetails

Target: Ig

Bindungsspezifität: Chain kappa

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Molecular Weight: 25 kDa.

Immunogen: Purified human Kappa light chain from human Bence Jones proteins

Anwendungsinformationen

Applikationshinweise: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil. Cellular Localization: Cytoplasmic.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulin Ig (Ig Produkte)

Hintergrund: Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin’s (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin. This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin’s lymphomas. In normal human serum lambda chains are present 1.6-15.2 mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient’s urine.