Kurzübersicht für Ziege anti-Human IgG Antikörper (Alkaline Phosphatase (AP)) (ABIN336602)
Target
IgG
Fragment
Fc fragment
Klonalität
Polyklonal
Konjugat
Alkaline Phosphatase (AP)
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)
Reaktivität
Human
Wirt
Ziege
Verwendungszweck
Goat anti-Human IgG Fc, Affinity Pure, ALP Conjugate, min x w/bovine, horse, mouse or rabbit serum proteins
Spezifität
Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG
Kreuzreaktivität (Details)
Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgG F(ab)'2 fragment • serum proteins from bovine, horse, mouse, or rabbit
Aufreinigung
Affinity purified using solid phase Human IgG
Reinheit
Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.5 mg/mL
Buffer
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.