Ziege anti-Human IgG Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN336567

Kurzübersicht für Ziege anti-Human IgG Antikörper (Alkaline Phosphatase (AP)) (ABIN336567)

Target: IgG

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Produktdetails

Reaktivität: Human

Wirt: Ziege

Verwendungszweck: Goat anti-Human IgG (H&L) - Affinity Pure, ALP Conjugate

Spezifität: Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG • light chains on all human immunoglobulins

Kreuzreaktivität (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins

Aufreinigung: Affinity purified using solid phase Human IgG

Reinheit: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Human IgG, whole molecule

Anwendungsinformationen

Kommentare:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody