Ziege anti-Human IgA Antikörper (Alkaline Phosphatase (AP))
Kurzübersicht für Ziege anti-Human IgA Antikörper (Alkaline Phosphatase (AP)) (ABIN336539)
Target
Klonalität
Konjugat
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Reaktivität
- Human
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Wirt
- Ziege
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Verwendungszweck
- Goat anti-Human IgA (i± chain) - Affinity Pure, ALP Conjugate
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Spezifität
- Based on IEP, this antibody reacts with: • heavy (i±) chains on human IgA
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Kreuzreaktivität (Details)
- Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins
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Aufreinigung
- Affinity purified using solid phase Human IgA
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Reinheit
- Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
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Immunogen
- Purified Human IgA, (i± chain)
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Kommentare
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ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.5 mg/mL
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Buffer
- 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
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Haltbarkeit
- 12 months
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- IgA
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Substanzklasse
- Antibody
Target
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