Esel anti-Ratte IgG Antikörper (HRP)

Produktnummer ABIN336493

Kurzübersicht für Esel anti-Ratte IgG Antikörper (HRP) (ABIN336493)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Produktdetails

Reaktivität: Ratte

Wirt: Esel

Verwendungszweck: Donkey anti-Rat IgG (H&L) - Affinity Pure, HRP Conjugate

Spezifität: Based on IEP, this antibody reacts with: • heavy (i³) chains on rat IgG • light chains on all rat immunoglobulins

Kreuzreaktivität (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin rat serum proteins

Aufreinigung: Affinity purified using solid phase Rat IgG

Reinheit: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Rat IgG, whole molecule

Anwendungsinformationen

Kommentare:

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Konzentration: 1.0 mg/mL

Buffer: PBS, 1 % BSA & 0.1 % proclin 150, contains 0.1 % (v/v) Proclin 150

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50 % glycerol and store at -20 °C as a liquid.

Lagerung: 4 °C

Informationen zur Lagerung: Store freeze-dried powder at 2-8 °C.

Antigendetails

Target: IgG

Substanzklasse: Antibody