Maus anti-Ente IgY Antikörper

Produktnummer ABIN305009

Kurzübersicht für Maus anti-Ente IgY Antikörper (ABIN305009)

Target: IgY

Klonalität: Monoklonal

Applikation: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Klon: 14A3

Isotyp: IgG1

Produktdetails

Reaktivität: Ente

Wirt: Maus

Spezifität: Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.

Keine Kreuzreaktivität: Huhn

Produktmerkmale: Antigene is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Aufreinigung: Affinity purified

Immunogen: Purified Peking duck yolk IgY.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Kommentare:

Target Species of Antibody: Duck

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Lagerung: -20 °C

Informationen zur Lagerung: Short term: -20°C
Long term: -20°C.

Antigendetails

Target: IgY

Substanzklasse: Antibody

Molekulargewicht: 25 kDa