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Maus anti-Human IgG (Fc Region) Antikörper

Name: Maus anti-Human IgG (Fc Region) Antikörper
Brand: antibodies-online
SKU: ABIN302085
Availability: InStock

ELISA, FACS, WB, ICC Monoclonal EM-07 IgG1 unconjugated

IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B). (Maus anti-Human IgG (Fc Region) Antikörper)

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (Maus anti-Human IgG (Fc Region) Antikörper)

FACS

Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (Maus anti-Human IgG (Fc Region) Antikörper)

FACS

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood). (Maus anti-Human IgG (Fc Region) Antikörper)

FACS

5 Abbildungen

Produktnummer ABIN302085

232,25 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 2 bis 4 Werktagen

Kurzübersicht für Maus anti-Human IgG (Fc Region) Antikörper (ABIN302085)

Target

IgG

Klonalität

Monoklonal

Applikation

2569
2482
2284
1814
1471
921
874
404
357
232
232
103
91
85
48
43
42
24
19
18
16
16
12
9
8
8
7
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1

ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC)

Klon

EM-07

Isotyp

IgG1

Bindungsspezifität
2906

375

346

154

131

88

67

29

28

28

17

5

5

2

1

1

1

1

1

1

1

1

1

1
Fc Region
Reaktivität
774

733

655

441

437

245

209

202

194

154

122

118

98

98

83

77

70

58

31

28

27

27

27

16

15

10

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1
Human
Wirt
1827

1732

611

405

149

79

30

25

20

20

18

12

11
Maus

Verwendungszweck

Anti-Hu IgG (Fc) Purified

Spezifität

The mouse monoclonal antibody EM-07 reacts with Fc part of human heavy chain of secreted IgG antibodies and with isolated Fc fragments. It does not react with IgG Fc domain in a complex of BCR (B cell antigen receptor).

Kreuzreaktivität (Details)

Human, Other not determined. The antibody EM-07 does not crossreact with IgM.

Produktmerkmale

The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

Fusion protein of human IgG Fc fragment.
Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG1 PE

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, FACS, WB, IF Multiclonal IgG2a Recombinant Antibody HRP

Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR)

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)
ELISA, WB Polyclonal IgG beta-Gal

Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)

Applikationshinweise

Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Target

IgG

Substanzklasse

Antibody

Hintergrund

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

Molekulargewicht

150 kDa

Gen-ID

35