Kaninchen anti-Schaf IgG (Fc Region) Antikörper (HRP)

Produktnummer ABIN301069

Kurzübersicht für Kaninchen anti-Schaf IgG (Fc Region) Antikörper (HRP) (ABIN301069)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Schaf

Wirt: Kaninchen

Spezifität: Anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab')2

Aufreinigung: Immunoaffinity purified

Immunogen: Sheep IgG Fc fragment.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000 - 1:8000), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Sheep

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Deionized water - do not add sodium azide.

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.

Antigendetails

Target: IgG

Substanzklasse: Antibody