Kurzübersicht für Ziege anti-Maus Ig Antikörper (Biotin) - Preadsorbed (ABIN2689598)
Target
Ig
Klonalität
Polyklonal
Konjugat
Biotin
Applikation
ELISA, Flow Cytometry (FACS)
Isotyp
Ig Fraction
Reaktivität
Maus
Wirt
Ziege
Produktmerkmale
This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected. Note: This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative. For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.