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Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (PE)

IHC, WB, FACS, FM Polyclonal IgG PE F(ab')2 fragment
Rockland
Produktnummer ABIN2669891
Hersteller Produkt- Nr.: 711-708-127

Kurzübersicht für Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (PE) (ABIN2669891)

Target

IgG

Fragment

F(ab')2 fragment

Klonalität

  • 3453
  • 232
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 422
  • 400
  • 274
  • 262
  • 235
  • 119
  • 104
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 40
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
PE

Applikation

  • 2121
  • 2052
  • 1899
  • 1873
  • 999
  • 919
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotyp

IgG
  • Bindungsspezifität

    • 2396
    • 517
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reaktivität

    • 753
    • 706
    • 608
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Kaninchen

    Wirt

    • 1787
    • 1583
    • 568
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Esel

    Hersteller Produkt- Nr.

    711-708-127

    Hersteller

    Rockland

    Verwendungszweck

    F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-adsorbed

    Kreuzreaktivität (Details)

    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

    Produktmerkmale

    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

    Aufreinigung

    F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    Immunogen

    Immunogen: Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.

    Immunogen Type: Native Protein

  • Applikationshinweise

    Application Note: F(ab')2 Rabbit IgG Phycoerythrin Conjugated Antibody was tested in WB, and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Flow Cytometry Dilution: 1:100 - 1:250 Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 IF Microscopy Dilution: 1:100 - 1:200 Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

    Konzentration

    0.5 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

    Haltbarkeit

    12 months
  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Rabbit IgG [H&L] Antibody Phycoerythrin conjugated Pre-adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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