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Ziege anti-Human IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
Produktnummer ABIN2669854
Hersteller Produkt- Nr.: 609-105-017
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2398
    • 514
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Fc Region
    Reaktivität
    • 686
    • 680
    • 575
    • 439
    • 381
    • 208
    • 195
    • 144
    • 127
    • 126
    • 107
    • 102
    • 96
    • 88
    • 84
    • 58
    • 48
    • 34
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 1719
    • 1431
    • 527
    • 208
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Ziege
    Klonalität
    • 3146
    • 175
    • 16
    • 1
    Polyklonal
    Konjugat
    • 588
    • 417
    • 416
    • 369
    • 273
    • 262
    • 235
    • 119
    • 85
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 44
    • 40
    • 39
    • 39
    • 36
    • 36
    • 34
    • 32
    • 31
    • 30
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 23
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    • 15
    • 15
    • 15
    • 15
    • 13
    • 10
    • 8
    Alkaline Phosphatase (AP)
    Applikation
    • 2041
    • 1987
    • 1838
    • 1780
    • 998
    • 920
    • 896
    • 487
    • 424
    • 149
    • 98
    • 64
    • 58
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Hersteller Produkt- Nr.
    609-105-017
    Hersteller
    Rockland
    Verwendungszweck
    Human IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against Ms Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins.  Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.
    Produktmerkmale
    Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterilität
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human IgG F(c) fragment
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Human IgG F(c) Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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