Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper (DyLight 488)
Kurzübersicht für Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper (DyLight 488) (ABIN1990978)
Target
Fragment
Klonalität
Konjugat
Isotyp
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Ratte
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Wirt
- Ziege
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Spezifität
- Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulins. No reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse
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Kreuzreaktivität (Details)
- min. cross-reactivity to human, mouse lgG
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Produktmerkmale
- DyLight® 488 has a maximum absorbance at 493 nm, Emax = 518 nm.
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Aufreinigung
- affinity purified goat IgG
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Immunogen
- Purified Rat IgG, whole molecule
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Applikationshinweise
- 1:20 - 1:2,000 for most applications
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Buffer
- Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
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Handhabung
- Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgG
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Substanzklasse
- Antibody
Target
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