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Maus anti-Human IgM Antikörper

ELISA, WB Monoclonal 22G1-H8 IgG2b unconjugated
Rockland
Produktnummer ABIN1607857
Hersteller Produkt- Nr.: 209-301-031

Kurzübersicht für Maus anti-Human IgM Antikörper (ABIN1607857)

Target

IgM

Klonalität

  • 548
  • 106
Monoklonal

Applikation

  • 390
  • 297
  • 295
  • 255
  • 183
  • 145
  • 91
  • 91
  • 64
  • 62
  • 54
  • 53
  • 47
  • 38
  • 18
  • 17
  • 10
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB)

Klon

22G1-H8

Isotyp

IgG2b
  • Reaktivität

    • 233
    • 170
    • 92
    • 42
    • 34
    • 34
    • 30
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 2
    Human

    Wirt

    • 373
    • 241
    • 92
    • 25
    • 14
    • 5
    • 4
    • 1
    • 1
    Maus

    Hersteller Produkt- Nr.

    209-301-031

    Hersteller

    Rockland

    Verwendungszweck

    Human IgM Fc5u Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5μ.  No reaction was observed against Human IgG, Human IgA, Human Fc, Human Fab or Human Fab2.

    Produktmerkmale

    Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

    Aufreinigung

    Anti-Human IgM Fc5μ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in mice.

    Immunogen Type: Native Protein

  • Applikationshinweise

    Application Note: Human IgM Fc5μ antibody has been tested by ELISA and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:1000 - 1:10000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    IgM

    Substanzklasse

    Antibody

    Hintergrund

    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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