Esel anti-Huhn IgY Antikörper (Agarose Beads)

Produktnummer ABIN1381671

Kurzübersicht für Esel anti-Huhn IgY Antikörper (Agarose Beads) (ABIN1381671)

Target: IgY

Konjugat: Agarose Beads

Applikation: Separation (Sep)

Produktdetails

Reaktivität: Huhn

Wirt: Esel

Aufreinigung: Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.

The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose.

Immunogen: A donkey was immunized with highly purified chicken IgY.

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Gel

Buffer: Phosphate buffered saline (PBS), pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Antigendetails

Target: IgY

Substanzklasse: Antibody