After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant and conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
The specific activity of the anti-BIG agarose was determined. For this lot, 1 mg bovine IgG bound per mL agarose.
Aufreinigung
egg yolk-derived purified IgY, affinity purified
Immunogen
Chickens were immunized with highly purified bovine IgG isolated from normal pooled bovine serum.
Applikationshinweise
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Gel
Buffer
Phosphate buffered saline (PBS), pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Do not freeze. Sensitive to light. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.