After multiple immunizations, eggs were collected and the IgY fraction prepared and the Fc specific antibodies immunoaffinity purified and conjugated to horseradish peroxidase.
Aufreinigung
egg yolk-derived purified IgY, affinity purified
Immunogen
Chickens were immunized with highly purified IgG from normal mouse serum.
ELISA, IHC, WB, DB
Polyclonal
IgG
unconjugated
Fab fragment
Applikationshinweise
This antibody is suitable for ELISA and blotting applications. Optimal working dilutions should be determined for your particular assay conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline, pH 7.2, 50 % glycerol
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
Biohazard Informations: This product contains thimerosal which is hazardous.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.