Maus anti-Human IgG1 (Hinge Region) Antikörper (PE)

Produktnummer ABIN135627

Kurzübersicht für Maus anti-Human IgG1 (Hinge Region) Antikörper (PE) (ABIN135627)

Target: IgG1

Klonalität: Monoklonal

Konjugat: PE

Applikation: ELISA

Klon: 4E3

Isotyp: IgG1

Produktdetails

Bindungsspezifität: Hinge Region

Reaktivität: Human

Wirt: Maus

Spezifität: Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA

Produktmerkmale: Mouse Anti-Human IgG1 Hinge-PE

Aufreinigung: Protein A affinity column

Immunogen: Unknown

Anwendungsinformationen

Applikationshinweise:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Purification - Reported in literature , Note - For direct immunostaining of IgG, positive cells in flow cytometry applications, clone HP, (SB Cat. No. ,) is recommended
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 5 g/mL AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use
Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen

Karsten, Mehta, Shin, Diefenbach, Slein, Karpinski, Irvine, Broge, Suscovich, Alter: "A versatile high-throughput assay to characterize antibody-mediated neutrophil phagocytosis." in: Journal of immunological methods, Vol. 471, pp. 46-56, (2019) (PubMed).

Violari, Cotton, Kuhn, Schramm, Paximadis, Loubser, Shalekoff, Da Costa Dias, Otwombe, Liberty, McIntyre, Babiker, Gibb, Tiemessen: "A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation." in: Nature communications, Vol. 10, Issue 1, pp. 412, (2019) (PubMed).

Boesch, Kappel, Mahan, Chu, Crowley, Osei-Owusu, Alter, Ackerman: "Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands." in: Biotechnology and bioengineering, Vol. 115, Issue 5, pp. 1265-1278, (2019) (PubMed).

Antigendetails

Target: IgG1

Substanzklasse: Antibody