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Ziege anti-Maus IgA, IgG, IgM Antikörper - Preadsorbed

Rockland 610-101-430 ELISA, IHC, WB, DB Polyclonal IgG unconjugated
Rockland
Produktnummer ABIN129740
Hersteller Produkt- Nr.: 610-101-430
  • Target Alle IgA, IgG, IgM Produkte
    IgA, IgG, IgM
    Reaktivität
    • 72
    • 48
    • 33
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Maus
    Wirt
    • 159
    • 55
    • 4
    • 3
    • 2
    Ziege
    Klonalität
    • 215
    • 2
    Polyklonal
    Applikation
    • 116
    • 112
    • 78
    • 77
    • 69
    • 56
    • 26
    • 23
    • 22
    • 22
    • 18
    • 17
    • 16
    • 15
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Hersteller Produkt- Nr.
    610-101-430
    Hersteller
    Rockland
    Verwendungszweck
    Mouse IgG IgA IgM (H&L) Pre-Adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse isotypes and chain combinations.  ELISA was used to confirm specificity at less than 1 % of the target signal.
    Produktmerkmale
    Anti-Mouse IgG IgA and IgM whole molecule antibody generated in goat detects specifically Mouse IgG IgA and IgM whole molecules. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Mouse IgG IgA IgM (H&L) Antibody is ideal for researchers in Cancer, Immunology, and Microbiology research.
    Aufreinigung
    Secondary Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities.
    Sterilität
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Mouse IgG IgA IgM antibody has been tested by ELISA and Dot Blot, and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000-1:90,000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.2 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    IgA, IgG, IgM
    Andere Bezeichnung
    IgG,IgA,IgM (IgA, IgG, IgM Produkte)
    Substanzklasse
    Antibody
    Hintergrund
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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