Maus anti-Huhn IgY Antikörper - Preadsorbed

Produktnummer ABIN126817

Kurzübersicht für Maus anti-Huhn IgY Antikörper - Preadsorbed (ABIN126817)

Target: IgY

Klonalität: Monoklonal

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: 9B4

Isotyp: IgG1

Produktdetails

Reaktivität: Huhn

Wirt: Maus

Aufreinigung: Preadsorption: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: Chicken egg yolk immunoglobulin (IgY) Molecular weight is 150 kDa

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2xPBS, 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 30 °C. Thawed aliquots may be stored at 0 °C up to 3 months.

Antigendetails

Target: IgY

Andere Bezeichnung: IgG + IgY

Hintergrund: Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y

Molekulargewicht: 150 kDa