Maus anti-Human IgE Antikörper

Produktnummer ABIN120643

Kurzübersicht für Maus anti-Human IgE Antikörper (ABIN120643)

Target: IgE

Klonalität: Monoklonal

Applikation: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Klon: BE5

Isotyp: IgG1

Produktdetails

Reaktivität: Human

Wirt: Maus

Aufreinigung: Precipitation methods and ion exchange chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE

Anwendungsinformationen

Applikationshinweise: ELISA. Flow Cytometry: 5 - 10 μg/mL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 4-8 °C for up to one month or at -28 °C for longer.

Antigendetails

Target: IgE

Hintergrund: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human

Molekulargewicht: 180 kDa