Maus anti-Ente IgY (Light Chain) Antikörper

Produktnummer ABIN120329

Kurzübersicht für Maus anti-Ente IgY (Light Chain) Antikörper (ABIN120329)

Target: IgY

Klonalität: Monoklonal

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 14A3

Isotyp: IgG1

Produktdetails

Bindungsspezifität: Light Chain

Reaktivität: Ente

Wirt: Maus

Produktmerkmale: Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. ELISA. Western Blot.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Antigendetails

Target: IgY

Andere Bezeichnung: IgG + IgY

Hintergrund: IgY Light Chain is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y

Molekulargewicht: 25 kDa