Ziege anti-Ratte Ig Antikörper (HRP) - Preadsorbed

Produktnummer ABIN1177364

Produktdetails

Target: Ig

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA

Marke: BD Pharmingen™

Aufreinigung: Preadsorption: Negative adsorption

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: This antibody conjugate has been tested by ELISA (1:1000 dilution). It is reactive in ELISA with rat IgGs, IgM, and IgA, a weaker signal may be detected on the rat IgG2b and IgM isotypes. It has some cross-reactivity with mouse IgG and no cross-reactivity with human Igs. For immunohistochemical staining (IHC) of acetone-fixed frozen and paraffin-embedded sections, we recommend the use of biotinylated goat anti-rat Ig in our special formulation for immunohistochemistry. However, when using rat IgG2b primary antibodies, we suggest using biotinylated mouse anti-rat IgG2b mAb G15-337 as second step in IHC. When using rat IgM primary antibodies in IHC, we recommend biotinylated anti-rat and -hamster Ig kappa light chain mAb RG7/7.6 or biotinylated anti-rat IgM mAb G53-238 as second step.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C. Avoid multiple freeze-thaws of product. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed. The antibody solution was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with mouse, human, cow, and horse serum proteins (multiple adsorption).

Antigendetails

Target: Ig

Abstract: Ig Produkte