Kaninchen anti-Human IgM (Fc5mu Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN117295

Kurzübersicht für Kaninchen anti-Human IgM (Fc5mu Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN117295)

Target: IgM

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Produktdetails

Bindungsspezifität: Fc5mu Region

Reaktivität: Human

Wirt: Kaninchen

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen: Human IgM (Fc5µ) fragment

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutesat room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C.

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Synonyms: Human Immunoglobulin M