Huhn anti-Human IgG (Whole Molecule) Antikörper (HRP) - Preadsorbed
Kurzübersicht für Huhn anti-Human IgG (Whole Molecule) Antikörper (HRP) - Preadsorbed (ABIN117235)
Target
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- Whole Molecule
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Reaktivität
- Human
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Wirt
- Huhn
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Aufreinigung
- Preadsorption: Solid phase absorption
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Immunogen
- Human IgG whole molecule
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Applikationshinweise
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Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- Restore with 1.0 mL of deionized water (or equivalent).
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Konzentration
- 1.0 mg/mL
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
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Konservierungsmittel
- Gentamicin sulfate
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Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handhabung
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- IgG
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Substanzklasse
- Antibody
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Hintergrund
- Synonyms: Human Immunoglobulin G
Target
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