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Ziege anti-Maus IgG3 Antikörper (FITC)

Rockland 610-102-043 WB, IHC, FACS, FLISA, DB, FM Polyclonal IgG FITC
Rockland
Produktnummer ABIN1118504
Hersteller Produkt- Nr.: 610-102-043
  • Target Alle IgG3 Produkte
    IgG3
    Reaktivität
    • 75
    • 19
    • 3
    Maus
    Wirt
    • 35
    • 34
    • 17
    • 10
    • 1
    Ziege
    Klonalität
    • 62
    • 26
    Polyklonal
    Konjugat
    • 19
    • 12
    • 12
    • 11
    • 7
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Applikation
    • 57
    • 41
    • 37
    • 29
    • 27
    • 18
    • 11
    • 11
    • 10
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)
    Hersteller Produkt- Nr.
    610-102-043
    Hersteller
    Rockland
    Verwendungszweck
    Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed
    Kreuzreaktivität (Details)
    Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
    Produktmerkmale
    Anti-Mouse IgG2b Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).
    Aufreinigung
    Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen

    Immunogen: Mouse IgG3 heavy chain

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Mouse IgG3 Fluorescein antibody has been tested by dot blot and is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. Flow Cytometry Dilution: 1:200 - 1:500 Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    IgG3
    Abstract
    IgG3 Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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